Published December 1980
| Version v1
Journal article
Absence of fractionation, protraction, radiation quality, and radical scavenger effects on radiation-induced interphase death of human G0 lymphocytes in vitro
Creators
- 1. Goettingen Univ. (Germany, F.R.). Inst. fuer Medizinische Physik und Biophysik
Description
In vitro interphase death of human peripheral blood lymphocytes, measured by shortening of the mean lifetime of small G0lymphocytes, was studied with 150 kV and 10 kV X-rays. No effect of radiation quality, of fractionation and of protraction was observed. The possibility of protection by L-cysteine and DMSO was tested in cells irradiated with 150 kV X-rays, but no protection effect with respect to cell survival could be demonstrated. Non-DNA radiation-sensitive site(s), possibly the cell membrane, appear to be responsible for interphase death. (orig.)
Abstract (German)
Der Interphasentod menschlicher, aus peripherem Blut entnommener Lymphozyten nach 150 kV- und 10 kV-Roentgenbestrahlung wurde durch Messung der mittleren Lebenszeitverkuerzung der kleinen Lymphozyten in der G0-Phase untersucht. Einfluesse der Strahlenqualitaet, der Fraktionierung und der Protahierung wurden nicht beobachtet. Die Moeglichkeit der Schutzwirkung durch L-Cystein un dDMSO wurde mit 150 kV-Roentgenstrahlung untersucht, aber in bezug auf das Zellueberleben konnte kein Schutzeffekt nachgewiesen werden. Der Interphasentod scheint nicht durch Wirkungen auf die DNS, sondern auf andere strahlenempfindliche Bereiche, moeglicherweise der Zellmembran, ausgeloest zu werden. (orig.)Additional details
Publishing Information
- Journal Title
- Strahlentherapie
- Journal Volume
- 156
- Journal Issue
- 12
- Series
- Strahlentherapie.
- Journal Page Range
- 839-844
- ISSN
- 0039-2073
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 13650195
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- CELL KILLING; CHRONIC IRRADIATION; CYSTEINE; DMSO; EFFICIENCY; FRACTIONATED IRRADIATION; IN VITRO; LIFETIME; LYMPHOCYTES; RADIATION INJURIES; RADIATION PROTECTION; RADIATION QUALITY; RADICALS; RADIOPROTECTIVE SUBSTANCES; RADIOSENSITIVITY; SCAVENGING; X RADIATION
- Descriptors DEC
- AMINO ACIDS; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; INJURIES; IONIZING RADIATIONS; IRRADIATION; LEUKOCYTES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RESPONSE MODIFYING FACTORS; SOMATIC CELLS; SULFOXIDES; THIOLS