Effect of 0.4 mT power frequency magnetic field on F-actin assembly of CHL cells
Creators
- 1. Key Laboratory of Optical and Magnetic Resonance Spectroscopy, East China Normal Univ., Shanghai (China)
Description
Objective: To investigate the effect of 0.4mT power frequency magnetic field on the microfilament (F- actin) assembly of Chinese hamster lung (CHL) cells. Methods: F-actin were marked with immunohistochemical method, then observed under a confocal microscope. The content of ECFRs in the preparation of the detergent-insoluble cytoskeleton was measured with Western-blotting. Results: The stress fiber's of CHL cells decreased after exposure to 0.4mT power frequency magnetic field for 30min, as well as after treatment with epidermal growth factor (ECF) of 50nM. Filopodias appeared at the periphery after exposure to magnetic field as well as treatment with EGF. The EGF receptor mass associated with the detergent-insoluble cytoskeleton increased after exposure to magnetic field as well as treatment with EGF. Conclusion: 0.4mT power frequency magnetic field induced assembly of F-actin in CHL cells. The change induced by magnetic field would be related to clustering of EGFR induced by magnetic field and passing the signal down. (authors)
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Health
- Journal Volume
- 16
- Journal Issue
- 3
- Journal Page Range
- p. 259-261
- ISSN
- 1004-714X
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 41105384
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ACTIN; BIOLOGICAL RADIATION EFFECTS; EPIDEMIOLOGY; FIBROBLASTS; GROWTH FACTORS; HAMSTERS; LUNGS; MAGNETIC FIELDS; MICROSCOPY; MICROTUBULES; RECEPTORS; SIGNALS
- Descriptors DEC
- ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BODY; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RESPIRATORY SYSTEM; RODENTS; SOMATIC CELLS; VERTEBRATES
Optional Information
- Notes
- 5 figs., 11 refs.