Mouse spleen lymphocyte bactericidal and peroxidase activites: enhancement by whole body X-irradiation

A 20,000 g pellet obtained from the homogenate of CD1 mouse spleen cells has measurable peroxidase and bactericidal activities. Whole body x-irradiation stimulates the in vitro peroxidative and bactericidal activities of the spleen cells. These cells do not phagocytize. However, addition of polystyrene latex particles to spleen cells from non- or x-irradiated mice, increases glucose oxidation. The extent of this stimulation (three- to fourfold) is less than that of PMN (six- to tenfold). Interaction between particles and lymphocyte surface may be the cause of this stimulation. The increased bactericidal activity due to x-ray is attribted mainly to an increase in the peroxidase activity of the lymphocytes. Surface (membrane) action rather that phagocytosis may be involved in the bactericidal process. The spleen lymphocyte peroxase is distinct from mylloproxidase in several resects