Action of carbamide, UV-radiation, and gamma-rays ob physico-chemical characteristics of native and immobilized inulinase

The stability of immolization inulinase to the medium various physicochemical factors is studied. The immobilization was accomplished on the ionites through the adsorption and modified glutaraldehyde methods. The chromatography, spectrophotometry and Laere general and modified methods were used by manufacture of preparation. By studying the stability of free and immobilized ferments the preparations were incubated with urea at 50 deg C and the substrate pH 4.7 during 20 minutes with subsequent determination of catalytic activity. the ferment was also subjected to single-time irradiation at a gamma-facility with the 60Co source by the dose rate of 1.36 Gy/min per cm2 in doses of 40-160 Gy. The inulinase UV-irradiation was performed with the dose rate of 150 J/m2 min. It is shown, that the ionite immobilization increases the inulinase stability to the denaturating agents. the character of bond with a carrier essentially effects the immobilized ferment stability to the effect of physical factors. The increase in the immobilized inulinase radioresistance is conditioned by multipoint interaction of the ferment molecule with the carrier active groups