Dynamic structure of protein (H-D exchange)

The dynamic structure of protein in solutions was examined by the hydrogen exchange method (H-D exchange). Of the various methods, the ultraviolet absorbance (fluorescence) stopped flow method was used. This method enables measurements of the exchange reaction of not only peptide hydrogen, byt also hydrogen on the side chain of protein. The reaction rate in solutions and the dynamic aspect of structure were reviewed with regard to erabutoxin b, α-subunit of tryptophan synthetase of E. coli and the tryptophan residue of muscular myosin. (Chiba, N.)