Synthesis of a carbon-14 labelled peptidyl carbamate human leukocyte elastase inhibitor and its immobilization on a tritiated water soluble polymer

A carbon-14 labelled hemisuccinate peptidyl human leukocyte elastase inhibitor, p-nitrophenyl-N-(1,4-[14C])-succinyl-L-alanyl-L-alanyl-prolylmethyl-N-isopropyl carbamate, and a tritium labelled hydrophilic polymer, poly-α, β-[N(2-hydroxyl-1-[3H]-ethyl)]-D, L aspartamide were prepared and are reported here. The immobilization of the [14C]-peptidyl carbamate onto the tritiated polymer to afford a double labelled macromolecular inhibitor of human leukocyte elastase is also illustrated. (author)