Published May 1991 | Version v1
Journal article Metadata-only

Radioimmunoassay of phenytoin

Creators

  • 1. Bhabha Atomic Research Centre, Bombay (India). Isotope Div.
  • 2. BRIT, New Bombay (India). Radiopharmaceuticals Operation
  • 3. International Atomic Energy Agency, Vienna (Austria). Chemistry and Industrial Application Section

Description

A radioimmunoassay procedure is described for the measurement of phenytoin (5,5-diphenylhydantoin) in serum samples. Antiserum to phenytoin was produced against phenytoin - valeric acid - bovine serum albumin conjugate. 125I labelled phenytoin - acetic acid - tyrosine methyl ester was used as a tracer. The assay covers a range of 10-500 ng/cm3 and has a sensitivity of 0.25 ng. The assay is validated by specificity tests, precision profile and recovery tests. (author) 8 refs.; 6 figs.; 2 tabs

Additional details

Publishing Information

Journal Title
Journal of Radioanalytical and Nuclear Chemistry
Journal Volume
149
Journal Issue
1
Series
J. Radioanal. Nucl. Chem. ;Articles.
Journal Page Range
3-11
ISSN
0236-5731
CODEN
JRNCD

INIS

Country of Publication
Hungary
Country of Input or Organization
Hungary
INIS RN
22071655
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACCURACY; ALBUMINS; CHEMICAL PREPARATION; IMMUNE SERUMS; IODINE 125; LABELLING; QUALITY CONTROL; RADIOIMMUNOASSAY; RADIOPHARMACEUTICALS; TRACER TECHNIQUES; TYROSINE; VALERIC ACID
Descriptors DEC
AMINO ACIDS; AROMATICS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CONTROL; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HYDROXY ACIDS; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MATERIALS; MONOCARBOXYLIC ACIDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; RADIOACTIVE MATERIALS; RADIOISOTOPES; SYNTHESIS