Nanoparticulate formulatiom of christmas palm seed (Adonidia merrillii) ethanolic extract containing lactic acid for antidiarrheal therapy

Preparation of Christmas Palm seeds ethanolic extract and lactic acid loaded nanoparticles aims to obtain an antidiarrheal (caused by Escherichia coli) therapy by using nano herbal. Preparation of the nanoparticles was done by ionic gelation method. There were three formulas (F) which had been evaluated in this study based on the difference amount of Ca(OH)₂, 12.5 mg for F1, 17.5 mg for F2, and 22.5 mg for F3. All formulas revealed an entrapment efficiency percentage of 75.489 over or less 0.563 (F1); 76.885 over or less 0.046 (F2); and 74.844 over or less 0.724% (F3). The second formula (F2) produced the highest %EE therefore it was distinguished as the optimum formula. The results of nanoparticles characterization of an optimum formula such as average diameter, poly dispersity index (PDI), and zeta potential using particle size analyzer were 1230.1 nm; 0.482; dan 27.5 mV respectively. An in-vivo antidiarrheal evaluation on rats using an optimum formula containing 1 gram of extract showed that nanoparticles possed an equal antidiarrheal effect to intraperitoneal gentamicin sulfate 8 mg/kg body weight with diarrhea inhibitory of 71.539%. (paper)