Accumulation of 10B-boronophpenylalanine in intracerebral implantation model of mouse melanoma cells

To construct an intracellular implantation model, human tyrosinase gene was transduced into achromatic melanoma cell (A1059) using plasmid (BCMGS-MHT-2). Suspension of the transgenic cell (TA1059), A105 or the normal cells was in vivo implanted into the right nucleus of basal ganglion in mouse brain. The accumulation capacity of fluorine-18-labeled fluoroboronophenylalanin (18F-10B-FBPA) was investigated with the intracerebral implantation model. The compound was intraperitoneally injected to those implanted mice and its accumulation was estimated by autoradiography. Either of A1059 and TA1059 cells was demonstrated to be implantable at the rate of 100% into the mouse brain. The surviving times of mice implanted with A1059 and TA1059 cells were 15.5±1.07 days and 21.7±1.28 days, respectively, showing that the surviving time of the latter was significantly longer. Both tumors derived from A1059 and TA1059 cells were strongly invasive and able to produce melanin even after the implantation. The incorporation of 18F-10B-FBPA was 3-4 times higher in the A1059 and TA1059 cells compared with that of the normal control, but the difference of incorporation between the two tumor cells were not significant. (M.N.)