Published October 2001 | Version v1
Journal article Metadata-only

Relationship between cell transformation and DNA strand breaks induced by α-radiation

Creators

  • 1. Academy of Military Medical Sciences, Beijing (China). Inst. of Radiation Medicine

Description

Objective: To investigate the quantitative relationship between HEL-9402 cell transformation and DNA strand breaks induced by α-particles. Methods: Nick translation assay was used to detect the level of DNA strand break. Cloning formation efficiencies in soft agar were used to evaluate cell transformation. Results: Level of DNA strand break and cloning formation efficiencies in soft agar increased obviously after HEL-9402 cells were irradiated by 0.25-5 Gy of α-particles, and there were statistical relationships between DNA strand breaks and cell transformation of irradiated HEL-9402. Conclusion: DNA damages are closely related to α particle irradiation-induced carcinogenesis

Additional details

Publishing Information

Journal Title
Chinese Journal of Radiological Medicine and Protection
Journal Volume
21
Journal Issue
5
Journal Page Range
p. 351-353
ISSN
0254-5098

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
33016661
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
ALPHA PARTICLES; DNA; EMBRYOS; LUNGS; MAN; PLUTONIUM 238; RADIATION INJURIES; RADIOINDUCTION; RESPIRATORY TRACT CELLS; STRAND BREAKS
Descriptors DEC
ACTINIDE NUCLEI; ALPHA DECAY RADIOISOTOPES; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BODY; CHARGED PARTICLES; DISEASES; DNA DAMAGES; EVEN-EVEN NUCLEI; HEAVY ION DECAY RADIOISOTOPES; HEAVY NUCLEI; INJURIES; IONIZING RADIATIONS; ISOTOPES; MAMMALS; NUCLEI; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PLUTONIUM ISOTOPES; PRIMATES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RESPIRATORY SYSTEM; SILICON 32 DECAY RADIOISOTOPES; SOMATIC CELLS; SPONTANEOUS FISSION RADIOISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES