Towards on-the-fly Cryo-Electron Microscopy Data Processing by High Performance Data Analysis

In this paper we present an approach to the on-the-fly Cryo-EM data acquisition and analysis using High Performance Data Analysis concept. An analysis of bottlenecks in the currently used data processing is presented, and the new iterative scheme for near real time implementation of the Cryo-EM data processing is discussed. The on-the-fly approach is proposed that will considerably reduce the time required for the protein (or other biological objects, e.g. viruses) 3D structure determination and allow eliminating manual interventions in the data processing. (paper)