Change of the NADPH depending superoxide producing and ferri hemoglobin reducing activities of cytochrome b558 from spleen cells and erythrocytes membranes induced by the radiation of different character

After the X radiation, UVA radiation and ultrasound radiation of new isoforms of cytochrome cyt b558 from rats erythrocyte membranes - EM (cyt b558III) and from spleen cell membranes (SCM) in vitro, as well as after the radiation of EM ex vivo, the suppression of both NADPH depending O2- producing and ferrihemoglobin (ferriHb)-reducing activities of cyt b558 from EM and SCM in homogeneous (in solution) and heterogeneous phases (in EM and SCM) at various scopes takes place. These changes are associated with the destabilization of EM and SCM, conditioned by the change of the aggregation degree of these hemoproteins in EM and SCM, hemoproteins as a result of the influence of the hydrogen peroxide formed during radiolysis and photolysis of the water medium. After He-Ne laser radiation of the cyt b558 from EM and SCM in vitro an increase of the NADPH depending O2- producing and ferriHb-reducing activities of the cyt b558 from EM and SCM in homogenous and heterogeneous phases (in membranes) takes place. It is supposed that the suppression (by X-, UVA- and US-radiation) and the stimulation (by He-Ne laser radiation) of the immune system activity and the oxygen homeostasis are associated with the corresponding decrease and increase of the NADPH depending O2- producing and ferriHb-reducings activity of the new isoforms of cyt b558 from EM and SCM in homogeneous and heterogeneous phases