Sorghum mutant lines characteristics based on in-vitro gas production and degradability value

Gas production techniques are used to compare sorghum mutant lines stover produced by BATAN as ruminants feed. 8 types of lines and complete randomized design with least significant difference (LSD) test was used to compare treatments. Samples were weighted 375 mg, put into 100 ml syringe glass and added by 30 ml bicarbonate buffer media then incubated at 39 ^oC for 24 hours. Parameters determined were gas production after 0, 2, 4, 6, 8,19, 12, 24 and 48 hours incubation, gas potential, dry matter (DBK) and organic matters (DBO) degradability. The result showed that gas production, gas potential and degradability were significantly different (P<0.05). The highest gas production after 24 and 48 hours was treatment H and B (57.59 and 71.75 ml / 375 mg BK), while the lowest was treatment I and G ( 50.33 and 54.92 ml / 375 mg BK). The highest gas production potential is treatment B (85.46 ml / 375 mg BK) and the lowest is G (56.74 ml / 375 mg BK), meanwhile the highest percentage of gas production during 24 hours is highest is treatment G (82.45 %) and the lowest is B (62.24 %). The highest degradability of dry matter (DBK) after 24 and 48 hours incubation was treatment D and A (54.45 and 67.31 %), while the lowest was treatment G (50.85 and 58.93 %). The highest DBO after 24 and 48 hour incubation was treatment D and B (54.13 and 69.13 %), while the lowest was treatment G (50.67 and 58.93 %). Generally there are two types of irradiated sorghum mutant lines that are easily degraded before 24 hours and after 24 hours, so they can be used for feeding guidelines for ruminants. (author)