Published December 1977 | Version v1
Journal article Metadata-only

Radioimmunoassay of enkephalins

Creators

  • 1. Max-Planck-Institut fuer Psychiatrie, Muenchen (Germany, F.R.). Abt. fuer Neuropharmakologie

Description

Using highly sensitive and highly specific antisera methionine- and leucine-enkephalin levels were determined in various areas of the rat brain. The highest content of both enkephalins was found in the striatum and the hypothalamus, whereas in the hippocampus, the cerebellum and the cortex only a low content was present. The ratio methionine-enkephalin/leucine-enkephalin was about three. Neither acute or chronic morphine treatment nor precipitated morphine withdrawal induced significant changes in enkephalin levels in any brain region. Also hypophysectomy did not affect the enkephalin content of the various brain regions. (orig.)

Abstract (German)

Unter Verwendung von hochempfindlichen und hochspezifischen Antiseren wurden Menthionin- und Leuzin-Enzephalin-Spiegel in verschiedenen Gebieten im Rattenhirn bestimmt. Der hoechste Gehalt an beiden Enzephalinen fand sich im Corpus striatum und im Hypothalamus, waehrend im Hippokampus, im Kleinhirn und im Kortex nur ein geringer Gehalt festgestellt wurde. Das Verhaeltnis Mentionin-Enzephalin zu Leuzin-Enzephalin war ungef. 1 zu 3. Weder akute noch chronische Morphium-Behandlg. noch beschleunigter Morphiumentzug bewirkten signifikante Veraenderungen des Enzephalin-Spiegels in irgeneiner Hirnregion. Auch die Hypophysenextirpation hatte keinen Einfluss auf den Enzephalingehalt der verschiedenen Hirnregionen. (orig.)

Additional details

Additional titles

Subtitle (English)
Regional distribution in rat brain after morphine treatment and hypophysectomy

Publishing Information

Journal Title
Arch. Pharmakol., Naunyn-Schmiedebergs
Journal Volume
301
Journal Issue
1
Series
Arch. Pharmakol., Naunyn-Schmiedebergs.
Journal Page Range
79-82

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
10445425
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
BRAIN; HYPOPHYSECTOMY; IMMUNE SERUMS; MORPHINE; PEPTIDES; RADIOIMMUNOASSAY; RATS; THERAPY; TRITIUM COMPOUNDS
Descriptors DEC
ALKALOIDS; ANALGESICS; ANIMALS; BODY; CENTRAL NERVOUS SYSTEM; DRUGS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; MEDICINE; NARCOTICS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SURGERY; TRACER TECHNIQUES; VERTEBRATES