Published November 1996 | Version v1
Journal article Metadata-only

Establishment of radioligand binding assay of gastrin receptors

Creators

  • 1. Shanghai Medical Univ. (China). Huashan Hospital

Description

Radioligand binding assay of gastrin receptor (GR) was established to determine GR content and Kd of gastric cancer and mucosa in specimens of 34 patients. Both of Gs-5 and [leu15]Gs-17, as unlabelled ligand, can inhibit the binding of 125I-(leu15)Gs-17 to the membrane, and the inhibiting effect of Gs-5 is slightly stronger than that of (leu15)Gs-17. Mixed cellulose acetate filters have better effect to separate bound 125I-(leu15)Gs-17 from free 125I-(leu15)Gs-17 than glass fibre filters (Hongguan 999999). The GR of gastric cancer correlates with that of mucosa in the same region of 34 patients

Additional details

Publishing Information

Journal Title
Nuclear Techniques
Journal Volume
19
Journal Issue
11
Journal Page Range
p. 653-656.
ISSN
0253-3219
CODEN
NUTEDL

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
28025272
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
CARCINOMAS; EXPERIMENTAL DATA; GASTRIN; IODINE 125; LABELLED COMPOUNDS; LIGANDS; RADIORECEPTOR ASSAY; RECEPTORS; STOMACH
Descriptors DEC
BETA DECAY RADIOISOTOPES; BODY; DATA; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; HORMONES; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NEOPLASMS; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES