Published June 10, 2013 | Version v1
Journal article Metadata-only

DNA damage in oral cancer cells induced by nitrogen atmospheric pressure plasma jets

Creators

  • 1. Department of Physics, University of Notre Dame, Notre Dame, Indiana 46556 (United States)
  • 2. Radiation Laboratory, University of Notre Dame, Notre Dame, Indiana 46556 (United States)
  • 3. Harper Cancer Research Institute, University of Notre Dame, Notre Dame, Indiana 46556 (United States)
  • 4. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 (United States)

Description

The nitrogen atmospheric pressure plasma jet (APPJ) was applied to induce DNA damage of SCC-25 oral cancer cells. Optical emission spectra were taken to characterize the reactive species produced in APPJ. In order to explore the spatial distribution of plasma effects, cells were placed onto photo-etched grid slides and the antibody H2A.X was used to locate double strand breaks of DNA inside nuclei using an immunofluorescence assay. The number of cells with double strand breaks in DNA was observed to be varied due to the distance from the irradiation center and duration of plasma treatment.

Additional details

Identifiers

DOI
10.1063/1.4809830;

Publishing Information

Journal Title
Applied Physics Letters
Journal Volume
102
Journal Issue
23
Journal Page Range
p. 233703-233703.5
ISSN
0003-6951
CODEN
APPLAB

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
44117455
Subject category
S60: APPLIED LIFE SCIENCES; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANTIBODIES; ATMOSPHERIC PRESSURE; BIOPHYSICS; DNA; EMISSION SPECTRA; IRRADIATION; MOLECULAR STRUCTURE; NEOPLASMS; NITROGEN; NUCLEI; ORAL CAVITY; PLASMA JETS; RADIOTHERAPY; SPATIAL DISTRIBUTION; STRAND BREAKS
Descriptors DEC
DIGESTIVE SYSTEM; DISEASES; DISTRIBUTION; DNA DAMAGES; ELEMENTS; MEDICINE; NONMETALS; NUCLEAR MEDICINE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHYSICS; RADIOLOGY; SPECTRA; THERAPY

Optional Information

Notes
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