Biofabrication of silver nanoparticles using Ostericum koreanum plant extract for treatment of allergic rhinitis in nursing care

In this work, the biofabrication of silver nanoparticles (Ag NPs) was carried out using leaf extract of Ostericum koreanum. The formation of crystalline Ag NPs was studied by using X-ray diffraction and UV–visible spectroscopy. HRTEM analysis confirmed the formation of spherical shaped Ag NPs with a mean size of 20 to 25 nm. FTIR spectroscopy confirmed the capping of Ag NPs with Ostericum koreanum extract polyphenols. On the other hand, Ag NPs were noticed to inhibit mRNA expressions and productions of inflammatory cytokines such as interleukin IL-6, (IL)-1β as well as tumor necrosis factor-α on phorbol 12-myristate 13-acetate plus A23187 (PMACI) stimulated human mast cell lines (HMC-1 cells). Ag NPs abolished the nuclear factor-κB and caspase-1 activations in HMC-1 cells, and decreased the expressions of IκB kinaseβ (IKKβ) and receptor interacting protein2 (RIP2). Additionally, Ag NPs increased the expression of IKKβ and RIP2 alone in regular condition. Ag NPs also inhibited the phosphorylation of protein kinase regulated by extracellular signal and anti-dinitrophenyl IgE-activated passive cutaneous anaphylaxis. The results of this work revealed that the biofabricated Ag NPs successfully enhanced the allergic inflammatory reaction of the mast cell implying their use for potential treatment of allergic diseases mediated by mast cell. (paper)