Radioisotope method for measuring photorespiration of plants

A method is suggested for direct measuring CO2 emission velocity from plant leaves and intracell decar boxylation velocity of photosynthesis intermediate products. On the basis of these factors interleave reassimilation can be calculated and carboxylation velocity can be determind. In measuring 14CO2 emission velocity in light, leaves are illucidated in 14CO2 medium for reaching photosynthesis stationary speed, and then they are exposed in the medium with 14CO2 up to the photorespiration substrate saturation by labell. After exposure in 14CO2 the chamber cleaning follows, carried out by its blowing with CO2 during 5S. After that the chamber is attached to a close system, in which 14CO2 radioactivity from a leaf is measured and recorded by a Geiger counter every 5S emitted. All the procedures described are carried out in a multichannel exposition chamber, providing a quick change of gaseous medium composition, around a leaf. In measuring 14CO2 emission from leaf experimental conditions during all 4 stages of the experiment and photosynthesis stationary state remain stable