Published May 1992 | Version v1
Journal article

Chemical characterization of complexation behavior of pertechnetate with cysteine

  • 1. National Tsing Hua Univ. Hsinchu (China). Inst. of Nuclear Science
  • 2. Institute of Nuclear Energy Research, Lung-Tan (China)

Description

The labeling behavior of cysteine with 99TcO4- ion and/or 99mTcO4- ion at different cysteine concentrations reductant and pH values has been studied by chromatography, and the labeling yield was calculated. Three major Tc-complexes, yellow, reddish brown and green can be separated by gel filtration chromatography (GFC). Thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and ion-exchange chromatography (IC) were used to separate the complexes collected from GFC. The TLC, HPLC data show the pertechnetate accompanied with a yellow complex; the green and purple complex contain more than two complexes. Electrophoresis and IC data show that the complexes carry a negative charge. The conductivity, UV-VIS, flow beta-detector with HPLC and autoradiography are also applied to analyze complex formation. (author) 20 refs.; 13 figs.; 5 tabs

Additional details

Publishing Information

Journal Title
Journal of Radioanalytical and Nuclear Chemistry
Journal Volume
159
Journal Issue
1
Journal Page Range
p. 129-144.
ISSN
0236-5731
CODEN
JRNCDM