Published June 2013 | Version v1
Journal article Metadata-only

Screening of strains with the high activity and thermostability nattokinase by 60Co γ-ray irradiation

Creators

  • 1. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing (China)
  • 2. College of Sciences, Henan Agricultural University, Zhengzhou (China)
  • 3. Agricultural Information Institute, Chinese Academic of Agricultural Sciences, Beijing (China)
  • 4. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou (China)

Description

In this study, Bacillus natto was irradiated by 60Co γ-ray, and activity was determined by Casein plate method in order to get high activity and thermostability strains. 60 strains with high activity were obtained through irradiation by 800 Gy 60Co γ-ray. In this dose, the positive mutation rate was 45%. Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃. (authors)

Additional details

Publishing Information

Journal Title
Journal of Nuclear Agricultural Sciences
Journal Volume
27
Journal Issue
6
Journal Page Range
p. 782-785
ISSN
1000-8551

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
48045539
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BACILLUS; BACILLUS SUBTILIS; CASEIN; COBALT 60; GAMMA RADIATION; IRRADIATION; MUTATIONS; PHOSPHOTRANSFERASES; RADIATION DOSES; RADIOINDUCTION; SCREENING; THERMAL DEGRADATION
Descriptors DEC
BACILLUS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; COBALT ISOTOPES; DOSES; ELECTROMAGNETIC RADIATION; ENZYMES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MICROORGANISMS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; RADIATIONS; RADIOISOTOPES; TRANSFERASES; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
4 figs., 11 refs.