Published 1993 | Version v1
Book

Recovery of 213Bi from an 225Ac cow: Application to the radiolabeling of monoclonal antibodies with the alpha-emitter 213Bi

  • 1. National Cancer Institute, Bethesda, MD (United States)
  • 2. Institute for Transuranium Elements, Karlsruhe (Germany)
  • 3. Alphamedical, Amsterdam (Netherlands)

Description

A column separation method was used to milk the α-emitting radionuclide, 213Bi, from an 225Ac cow: Milking was done on a 150 mm3 column of MP-50 cation exchange resin with the acids HI or HCl or both. To assay the complexation behavior of 213Bi, thin-layer chromatography was used with μM amounts of DTPA complexing ligand. Radiolabeling was done with the B3 monoclonal antibody (mAb) conjugate of the novel ligand CHX-B DTPA. The optimal results to-date are 38 μCi (76%) of 50 μCi213Bi incorporated into the mAb conjugate. The final specific activity was 0.24 μCi/μg. These results are discussed and some comparisons to the 212Bi system are made

Additional details

Publishing Information

Publisher
American Chemical Society.
Imprint Place
Washington, DC (United States)
Imprint Title
206th ACS national meeting
Imprint Pagination
1905 p.
Journal Page Range
p. 873, Paper NUCL 94.

Conference

Title
206. American Chemical Society (ACS) national meeting.
Dates
22-27 Aug 1993.
Place
Chicago, IL (United States).

Optional Information

Secondary number(s)
CONF-930802--.