Published February 8, 2008
| Version v1
Journal article
Identification of poly(ADP-ribose) polymerase-1 as the OXPHOS-generated ATP sensor of nuclei of animal cells
- 1. Department of Cellular and Molecular Pharmacology, University of California San Francisco, School of Medicine, San Francisco, CA 94143 (United States)
- 2. UCSF Helen Diller Family Comprehensive Cancer Center, Department of Anatomy, University of California, School of Medicine, San Francisco Medical Center, San Francisco, CA 94143 (United States)
- 3. Department of Medical Biochemistry, Semmelweis University, Budapest (Hungary)
Description
Our results show that in the intact normal animal cell mitochondrial ATP is directly connected to nuclear PARP-1 by way of a specific adenylate kinase enzymatic path. This mechanism is demonstrated in two models: (a) by its inhibition with a specific inhibitor of adenylate kinase, and (b) by disruption of ATP synthesis through uncoupling of OXPHOS. In each instance the de-inhibited PARP-1 is quantitatively determined by enzyme kinetics. The nuclear binding site of PARP-1 is Topo I, and is identified as a critical 'switchpoint' indicating the nuclear element that connects OXPHOS with mRNA synthesis in real time. The mitochondrial-nuclear PARP-1 pathway is not operative in cancer cells
Availability note (English)
Available from http://dx.doi.org/10.1016/j.bbrc.2007.12.004Additional details
Identifiers
- DOI
- 10.1016/j.bbrc.2007.12.004;
- PII
- S0006-291X(07)02622-8;
Publishing Information
- Journal Title
- Biochemical and Biophysical Research Communications
- Journal Volume
- 366
- Journal Issue
- 2
- Journal Page Range
- p. 568-573
- ISSN
- 0006-291X
- CODEN
- BBRCA9
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 39062923
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ADP; ANIMAL CELLS; ATP; BIOSYNTHESIS; CELL NUCLEI; INHIBITION; MITOCHONDRIA; NEOPLASMS; POLYMERASES; RIBOSE; TRIOCTYLPHOSPHINE OXIDE
- Descriptors DEC
- ALDEHYDES; CARBOHYDRATES; CELL CONSTITUENTS; DISEASES; ENZYMES; MONOSACCHARIDES; NUCLEOTIDES; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; PENTOSES; PHOSPHINE OXIDES; PHOSPHINES; PHOSPHORUS COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; SACCHARIDES; SYNTHESIS; TRANSFERASES
Optional Information
- Copyright
- Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.