A role for scavenger receptor B-I in selective transfer of rhodamine-PE from liposomes to cells
- 1. Department of Pathology and Laboratory Medicine, Medical Biology Section, Groningen University Institute for Drug Exploration (GUIDE), University of Groningen (Netherlands)
- 2. Department of Pharmacokinetics and Drug Delivery, Groningen University Institute for Drug Exploration (GUIDE), University of Groningen (Netherlands)
- 3. Department of Cell Biology, Groningen University Institute for Drug Exploration (GUIDE), University of Groningen (Netherlands)
Description
We investigated the potential role of scavenger receptor B-I (SR-BI) in the selective removal of liposomal markers from blood by hepatocytes. Liposomes were labeled with [3H]cholesteryloleyl-ether ([3H]COE), 1,2-di[1-14C]palmitoyl-phosphatidylcholine ([14C]PC), and N-(lissamine rhodamine-B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). The radiolabels were eliminated at identical rates from plasma, while N-Rh-PE was cleared twice as fast. Involvement of SR-BI in the selective removal of N-Rh-PE from liposomes was studied in transfected Chinese hamster ovary cells over-expressing SR-BI. Uptake of N-Rh-PE from liposomes containing phosphatidylserine was higher than [3H]COE, and was further enhanced by apolipoprotein A-I, confirming involvement of SR-BI in the selective uptake of liposomal N-Rh-PE by cells
Additional details
Identifiers
- DOI
- 10.1016/j.bbrc.2004.10.104;
- PII
- S0006-291X(04)02412-X;
Publishing Information
- Journal Title
- Biochemical and Biophysical Research Communications
- Journal Volume
- 325
- Journal Issue
- 3
- Journal Page Range
- p. 908-914
- ISSN
- 0006-291X
- CODEN
- BBRCA9
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 36058377
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BLOOD; CARBON 14; CHO CELLS; ETHERS; LECITHINS; LIPOSOMES; LIVER CELLS; RECEPTORS; TRITIUM; UPTAKE
- Descriptors DEC
- ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BODY FLUIDS; CARBON ISOTOPES; ESTERS; EVEN-EVEN NUCLEI; HYDROGEN ISOTOPES; ISOTOPES; LIGHT NUCLEI; LIPIDS; MATERIALS; MEMBRANE PROTEINS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHOLIPIDS; PROTEINS; RADIOISOTOPES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES
Optional Information
- Copyright
- Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.