Studying the ability of mutation and selection of promising lines of Brassia Verrucosa Lindl. by gamma irradiation in combination with in vitro technique

The objective of this study was to induce mutation in Warty Brassia (Brassia verrucosa Lindl.) through in vitro mutagenesis by treating the bud clusters with gamma irradiation, aiming to select the valuable varieties of Warty Brassia. The following contents were conducted: detecting of genetic polymorphism of the variants by applying RAPD analysis; evaluating the growth of variation forms of Warty Brassia obtaining after irradiation in the nursery stage; selecting desired variations. The results revealed that 9 variants were examined genetic diversity, 8 variants there were genetic differences among 8 variants and original variety. There were differences in the growth potential among morphological variants under nursery condition. The mutants having large pseudobulb, dark green leaves and the mutants having pseudobulb round in shape, short leaves promise to develop mutants strains. Types of mutation were selected and multiply rapidly from generation M₁V₁ - M₁V₃, they retained 100% morphological features of their parents. The study is being conducting to assess the flower color and shape of the mutants derived from Warty Brassia. (author)