Development of integrated in vivo/in vitro system for the study of carcinogenic effects of energy-related by-products. Progress report No. 2, November 1, 1978-October 31, 1979

Progress is reported in the following areas of research: development of a means of isolating type II cells from the mouse lung in a reproductively intact condition; growth of these cells in monolayer culture; serial propagation of these cells; production of transformants in vitro by the addition of carcinogens to the cultures; induction of transformation in vivo followed by assay in vitro; verification of the tumorgenic potential of the transformants by analysis of their quantitative and qualitative characteristics; quantitation of the in vivo and in vitro transformation process; and comparison of the quantitative aspects of the two systems with each other and with in vivo tumor yield patterns. Research in the first four areas was extended during the past year to include human lung cells, and on a limited scale, to include in vitro analysis of 131I radiation carcinogenesis in thyroid cultures