A blocking ELISA for detecting antibodies against Ausjeszky's disease virus and its application in serological screening
- 1. Sichuan Agricultural Univ., Ya'an, Sichuan (China). Isotope Research Lab.
Description
A blocking ELISA was developed for detecting antibodies in pigs exposed to Aujeszky's disease virus (ADV). This test was evaluated against the virus neutralization test and compared with a blocking ELISA supplied by the Joint FAO/IAEA Division. The blocking ELISA developed in this study had a similar sensitivity and specificity to the FAO/IAEA-ELISA when compared with the VN test. The sensitivity was 80.6% and the specificity was 95.2% when compared to the VN test. This blocking ELISA was used for a survey to determine the prevalence of Aujesky's disease in Sichuan Province. There was a high prevalence of infection in piggeries in Sichuan, suggesting that control by vaccination would be necessary. A proposed control strategy for Aujeszky's disease is discussed. (author). 16 refs, 3 tabs
Additional details
Publishing Information
- Imprint Title
- Strengthening research on animal reproduction and disease diagnosis in Asia through the application of immunoassay techniques
- Imprint Pagination
- 238 p.
- Journal Page Range
- p. 159-165.
- ISSN
- 1011-4289
- Report number
- IAEA-TECDOC--736
Conference
- Title
- Final research co-ordination meeting on strengthening research on animal reproduction and disease diagnosis in Asia through the application of immunoassay techniques.
- Dates
- 1-5 Feb 1993.
- Place
- Bangkok (Thailand).
INIS
- Country of Publication
- International Atomic Energy Agency (IAEA)
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 25049557
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference, Numerical Data
- Descriptors DEI
- ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; DIAGNOSTIC TECHNIQUES; ENZYME IMMUNOASSAY; EXPERIMENTAL DATA; PREVENTIVE MEDICINE; SWINE; VACCINES; VIRAL DISEASES; VIRUSES
- Descriptors DEC
- ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; DATA; DISEASES; DOMESTIC ANIMALS; IMMUNOASSAY; INFECTIOUS DISEASES; INFORMATION; MAMMALS; MATERIALS; MEDICINE; MICROORGANISMS; NUMERICAL DATA; PARASITES; VERTEBRATES