Three-dimensional structure of the enzyme dimanganese catalase from thermus thermophilus at 1 A resolution
Creators
- 1. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 117333 (Russian Federation)
- 2. European Molecular Biology Laboratory, EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, Hamburg, 22603 (Germany)
- 3. Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, P.O. Box 594, First Court, Western Bank, Sheffield, S10 2TN (United Kingdom)
Description
The crystal structures of two forms of the enzyme dimanganese catalase from Thermus Thermophilus (native and inhibited by chloride) were studied by X-ray diffraction analysis at 1.05 and 0.98 A resolution, respectively. The atomic models of the molecules were refined to the R factors 9.8 and 10%, respectively. The three-dimensional molecular structures are characterized in detail. The analysis of electron-density distributions in the active centers of the native and inhibited enzyme forms revealed that the most flexible side chains of the amino acid residues Lys162 and Glu36 exist in two interrelated conformations. This allowed us to obtain the structural data necessary for understanding the mechanism of enzymatic activity of the dimanganese catalase
Additional details
Identifiers
- DOI
- 10.1134/1.171145;
Publishing Information
- Journal Title
- Crystallography Reports
- Journal Volume
- 45
- Journal Issue
- 1
- Journal Page Range
- p. 105-116
- ISSN
- 1063-7745
- CODEN
- CYSTE3
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 35081533
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; S36: MATERIALS SCIENCE; S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Translation
- Descriptors DEI
- ATOMIC MODELS; CATALASE; CRYSTAL STRUCTURE; ELECTRON DENSITY; MOLECULAR STRUCTURE; R FACTORS; X-RAY DIFFRACTION
- Descriptors DEC
- COHERENT SCATTERING; DIFFRACTION; ENZYMES; MATHEMATICAL MODELS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PEROXIDASES; PROTEINS; SCATTERING
Optional Information
- Notes
- Translated from Kristallografiya, ISSN 0023-4761, 45, 111-122 (No. 1, 2000); (c) 2000 MAIK ''Nauka / Interperiodica''.