The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold
Creators
- Das, Debanu1, 2
- Kozbial, Piotr3, 2
- Han, Gye Won4, 2
- Carlton, Dennis4, 2
- Jaroszewski, Lukasz5, 3, 2
- Abdubek, Polat6, 2
- Astakhova, Tamara5, 2
- Axelrod, Herbert L.1, 2
- Bakolitsa, Constantina3, 2
- Chen, Connie6, 2
- Chiu, Hsiu-Ju1
- Chiu, Michelle6, 2
- Clayton, Thomas4, 2
- Deller, Marc C.4, 2
- Duan, Lian5, 2
- Ellrott, Kyle5, 2
- Elsliger, Marc-André4, 2
- Ernst, Dustin6, 2
- Farr, Carol L.4, 2
- Feuerhelm, Julie6, 2
- Grzechnik, Anna4, 2
- Grant, Joanna C.6, 2
- Jin, Kevin K.1, 2
- Johnson, Hope A.4, 2
- Klock, Heath E.6, 2
- Knuth, Mark W.6, 2
- Krishna, S. Sri5, 3, 2
- Kumar, Abhinav1, 2
- Marciano, David4, 2
- McMullan, Daniel6, 2
- Miller, Mitchell D.1, 2
- Morse, Andrew T.5, 2
- Nigoghossian, Edward6, 2
- Nopakun, Amanda4, 2
- Okach, Linda6, 2
- Oommachen, Silvya7, 8
- Paulsen, Jessica9, 8
- Puckett, Christina9, 8
- Reyes, Ron7, 8
- Rife, Christopher L.7, 8
- Sefcovic, Natasha10, 8
- Tien, Henry J.11, 8
- Trame, Christine B.7, 8
- Bedem, Henry van den7, 8
- Weekes, Dana10, 8
- Wooten, Tiffany9, 8
- Xu, Qingping7, 8
- Hodgson, Keith O.12, 8
- Wooley, John13, 8
- Deacon, Ashley M.7, 8
- Godzik, Adam13, 10, 8
- Lesley, Scott A.9, 11, 8
- Wilson, Ian A.11, 8
- 1. Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California (United States)
- 2. Joint Center for Structural Genomics, http://www.jcsg.org (United States)
- 3. Program on Bioinformatics and Systems Biology, Burnham Institute for Medical Research, La Jolla, California (United States)
- 4. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California (United States)
- 5. Center for Research in Biological Systems, University of California, San Diego, La Jolla, California (United States)
- 6. Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, California (United States)
- 7. Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California (US)
- 8. Joint Center for Structural Genomics, http://www.jcsg.org (US)
- 9. Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, California (US)
- 10. Program on Bioinformatics and Systems Biology, Burnham Institute for Medical Research, La Jolla, California (US)
- 11. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California (US)
- 12. Photon Science, SLAC National Accelerator Laboratory, Menlo Park, California (US)
- 13. Center for Research in Biological Systems, University of California, San Diego, La Jolla, California (US)
Description
KPN03535 is a protein unique to K. pneumoniae. The crystal structure reveals that KPN03535 represents a novel variant of the OB-fold and is likely to be a DNA-binding lipoprotein. KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family
Availability note (English)
Available from http://dx.doi.org/10.1107/S1744309109018168; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954213Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954213;
- DOI
- 10.1107/S1744309109018168;
- PII
- S1744309109018168;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 66
- Journal Issue
- Pt 10
- Journal Page Range
- p. 1254-1260
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46072678
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CRYSTAL STRUCTURE; DNA; PROTEINS
- Descriptors DEC
- NUCLEIC ACIDS; ORGANIC COMPOUNDS
Optional Information
- Copyright
- Copyright (c) Das et al. 2010
- Notes
- PMCID: PMC2954213; PMID: 20944219; PUBLISHER-ID: wd5110; OAI: oai:pubmedcentral.nih.gov:2954213; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.