Published 1979 | Version v1
Report

Tyhmidine synchronisation and chromosome labelling

Description

A technique is described for the blocking of cell cycle in the S phase with thymidine in final concentration of 0.3mg per ml. This permits a large number of mitotic cells to be obtained in the first generation and it promotes the accumulation of other forms (prophases, prometaphases, metaphases and anaphases) depending on the time of elimination of thymidine and of the fixation. (author)

Additional details

Additional titles

Original title (French)
Synchronisation par la thymidine et marquage chromosomique

Publishing Information

Imprint Title
Routine use of chromosome analysis in radiation protection
Journal Page Range
p. 123-128.
Report number
IAEA-TECDOC--224

Conference

Title
Advisory group meeting on routine use of chromosome analysis in radiation protection.
Dates
5 - 8 Dec 1978.
Place
Fontenay-aux-Roses, France.

INIS

Country of Publication
International Atomic Energy Agency (IAEA)
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
11554814
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Conference
Descriptors DEI
CELL CYCLE; CHROMOSOMES; SYNCHRONIZATION; THYMIDINE; TIME DEPENDENCE
Descriptors DEC
AZINES; HETEROCYCLIC COMPOUNDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES