Published 1976 | Version v1
Book Metadata-only

Estimation of pre-meiotic DNA synthesis period in the dog spermatozoa

Creators

  • 1. Burdwan Univ., West Bengal (India)
  • 2. Rhode Island Hospital, Providence (USA)

Description

About 10 μCi of 3H-thymidine was injected into each of 4 arbitarary sites in each testis of 6 dogs. Biopsies were taken at 4-hour intervals coverning a period from 20.0 to 22.2 days post-injection. Kinetics of labelled spermatocytes was followed employing Kodak NTB-3 emulsion to conventionally prepared air-dried slides. The technique for calculating pre-meiosis DNA synthesis duration is same as that for estimating S period in mitotic cells. Current investigation suggests that the mean duration of pre-meiotic S period of Canine spermatocytes is 20.4 hrs as compared to 29 and 40 hrs in spermatocytes of mouse and golden hamster respectively. (author)

Additional details

Publishing Information

Publisher
Department of Atomic Energy.
Imprint Place
Bombay
Imprint Title
Use of radiations and radioisotopes in studies of animal production, proceedings of a symposium held at Izatnagar, December 16-18, 1975
Journal Page Range
p. 284-288.

Conference

Title
Symposium on use of radiations and radioisotopes in studies of animal production.
Dates
16 - 18 Dec 1975.
Place
Izatnagar, India.

INIS

Country of Publication
India
Country of Input or Organization
India
INIS RN
9357637
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Resource subtype / Literary indicator
Conference
Descriptors DEI
BIOPSY; BIOSYNTHESIS; DNA; DOGS; LABELLED COMPOUNDS; MEIOSIS; SPERMATOCYTES; SPERMATOGENESIS; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS
Descriptors DEC
ANIMALS; AZINES; CELL DIVISION; DIAGNOSTIC TECHNIQUES; GAMETOGENESIS; GERM CELLS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIOACTIVE MATERIALS; RIBOSIDES; SYNTHESIS; VERTEBRATES

Optional Information

Notes
6 refs.