Published July 26, 2006 | Version v1
Journal article Metadata-only

Crystallization and initial X-ray analysis of polyhydroxyalkanoate granule-associated protein from Aeromonas hydrophila

Creators

  • 1. MOE Key Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084 (China)
  • 2. Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong (China)

Description

The phasin PhaPAh from A. hydrophila strain 4AK4 was crystallized using the hanging-drop vapour-diffusion method. Polyhydroxyalkanoate (PHA) granule-associated proteins (phasins) were discovered in PHA-accumulating bacteria. They play a crucial role as a structural protein during initial PHA-granule formation and granule growth and also serve as interfaces for granule stabilization in vivo. The phasin PhaPAh from Aeromonas hydrophila strain 4AK4 was crystallized using the hanging-drop vapour-diffusion method. Single crystals were cryocooled for X-ray diffraction analysis. The phasin crystals belonged to space group P212121, with unit-cell parameters a = 80.8, b = 108.9, c = 134.4 Å

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309106025000; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242923

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242923;
DOI
10.1107/S1744309106025000;
PII
S1744309106025000;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
62
Journal Issue
Pt 8
Journal Page Range
p. 814-819
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46065460
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; DIFFUSION; IN VIVO; INTERFACES; MONOCRYSTALS; PROTEINS; SPACE GROUPS; STABILIZATION; STRAINS; X-RAY DIFFRACTION
Descriptors DEC
COHERENT SCATTERING; CRYSTALS; DIFFRACTION; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; SCATTERING; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2006
Notes
PMCID: PMC2242923; PMID: 16880566; PUBLISHER-ID: gj5003; OAI: oai:pubmedcentral.nih.gov:2242923