Published 1982 | Version v1
Miscellaneous

Enzymic synthesis of nucleotides labelled with 32P

  • 1. Magyar Tudomanyos Akademia, Budapest. Izotopintezete

Description

A method of enzymic synthesis of nucleotides labelled with 33P is described. A considerable decrease of inorganic phosphate contaminations in the applied nucleotides of high specific activity has been achieved by means of enzymic purification using materials involved in ATP synthesis as well as glucose and hexokinase. This method permitted to simplify the preparation of nucleotides with 37 TBq/mmole specific activity and higher

Additional details

Additional titles

Original title (Russian)
Получение нуклеотидов, меченных

Publishing Information

Imprint Title
Radioisotope-labelled organic compounds. Part 2
Journal Page Range
p. 137-140.
Report number
INIS-mf--10548

Conference

Title
Radioisotope-labelled organic compounds.
Dates
8-11 Dec 1981.
Place
Leningrad (USSR).

INIS

Country of Publication
International Centre for Scientific and Technical Information (ICSTI)
Country of Input or Organization
International Centre for Scientific and Technical Information (ICSTI)
INIS RN
18008241
Subject category
S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Conference
Descriptors DEI
ENZYMES; LABELLING; NUCLEOTIDES; PHOSPHORUS 32; PURIFICATION; RADIOACTIVITY; SYNTHESIS
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES

Optional Information

Notes
Imprint:Organicheskie soedineniya, mechennye radioaktivnymi izotopami.;Materialy 2 Simpoziuma stran-chlenov SEhV, Leningrad, 8-11 dekabrya 1981 g. Chast'2.