Published June 2004 | Version v1
Journal article Metadata-only

Exploitation of the Dose/Time-Response Relationship for a New Measure of DNA Repari in the Single-Cell Gel Electrophoresis (Comet) Assay

Creators

  • 1. Yonsei University, Seoul (Korea, Republic of)
  • 2. University of Heidelberg (Germany)
  • 3. St. Vincentius Clinics, Karlsruhe (Germany)
  • 4. Municipal Clinics GmbH Karlsruhe (Germany)
  • 5. Surgical Department of the Heilbronn Clinics, Heilbronn (Germany)
  • 6. German cancer Research Center, Heidelberg (Germany)

Description

The comet assay (also called the single-cell gel electrophoresis assay) has been widely used for detecting DNA damage and repair in individual cells. Since the conventional methods of evaluating comet assay data using frequency statistics are unsatisfactory we developed a new quantitative measure of DNA damage/repair that is based on all information residing in the dose/time-response curves of a comet experiment. Blood samples were taken from 25 breast cancer patients before undergoing radiotherapy. The comet assay was performed under alkaline conditions using isolated lymphocytes

Additional details

Publishing Information

Journal Title
Journal of Toxicology and Public Health (Seoul)
Journal Volume
20
Journal Issue
2
Journal Page Range
p. 89-100
ISSN
1226-8399

INIS

Country of Publication
Food and Agriculture Organization of the United Nations (FAO)
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46021116
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL REPAIR; DNA; DNA DAMAGES; ELECTROPHORESIS; GELS; LYMPHOCYTES; MAMMARY GLANDS; NEOPLASMS; PATIENTS; PETROLEUM RESIDUES; RADIATION DOSES; RADIOTHERAPY; RESOURCE EXPLOITATION
Descriptors DEC
ANIMAL CELLS; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; COLLOIDS; CONNECTIVE TISSUE CELLS; DISEASES; DISPERSIONS; DOSES; ENERGY SOURCES; FOSSIL FUELS; FUELS; GLANDS; LEUKOCYTES; MATERIALS; MEDICINE; NUCLEAR MEDICINE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PETROLEUM; PETROLEUM FRACTIONS; RADIOLOGY; REPAIR; SOMATIC CELLS; THERAPY

Optional Information

Notes
FAO/AGRIS record; ARN: KR2005011020