Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation

Objective: To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation. Methods: AHH-1 cells was exposed to different doses(0, 1, 3, 5, 8, 10, 15 and 18 Gy)of ⁶⁰Co γ-rays, and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4, 8, 12, 24, 48 and 72 h after irradiation. Results: Within the range of applied doses, the level of S100A4 gene expression was up-regulated with a good dose-response (R² = 0.79 - 0.93, P < 0.05) and had obvious difference at different time points (F = 8.91, P < 0.01). Conclusion: S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation, and hence is applicable as a dosimeter. (authors)