Relative gene in immortalized human fetal tracheal fibroblasts malignantly transformed by alpha-particle irradiation

Objectives: To explore the relative novel genes which are associated with radiation-induced neoplastic transformation of cells. Methods: The experiment was carried out by reverse transcription of mRNA from SHTF and α SHTF cells, amplification of cDNA by PCR using 24 pairs of primers consisting of 3'[HT11M(G,C,A)] and arbitrary 5' (HAP17∼24) primers, tracing with 32P-dATP, running on 6% sequencing gels in adjacent lanes, and then autoradiographing. Results: Twenty-three DNA segments showed expression in α SHTF cells, but not in SHTF cells. By reamplification of 12 differential expression DNA segments of them at random, 6 DNA segments were obtained. Northern dots showed that C17-5 and C23-1 had positive responses in α SHTF cells, but negative in SHTF cells. Conclusions: The research indicates that there is difference in gene expression between SHTF cells and α SHTF cells