Published August 31, 2005 | Version v1
Journal article Metadata-only

Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans

Creators

  • 1. Institut de Recherches Microbiologiques JM Wiame (Belgium)
  • 2. Centre d'Etude Nucléaire, Mol-Boeretang (Belgium)
  • 3. Laboratoire de Microbiologie, Université Libre de Bruxelles (Belgium)
  • 4. Université de Mons-Hainaut (Belgium)
  • 5. University of Namur (Belgium)

Description

Overexpression, purification and crystallization of C. metallidurans CopK allowed the collection of a complete data set to 2.2 Å resolution. CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 Å, and diffract to 2.2 Å resolution using synchrotron radiation

Availability note (English)

Available from http://dx.doi.org/10.1107/S174430910502316X; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978110

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978110;
DOI
10.1107/S174430910502316X;
PII
S174430910502316X;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
61
Journal Issue
Pt 9
Journal Page Range
p. 825-827
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46061334
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CITRATES; COPPER; CRYSTALLIZATION; CRYSTALS; DIFFUSION; GLYCEROL; PROTEINS; RESOLUTION; SYMMETRY; SYNCHROTRON RADIATION
Descriptors DEC
ALCOHOLS; BREMSSTRAHLUNG; CARBOXYLIC ACID SALTS; ELECTROMAGNETIC RADIATION; ELEMENTS; HYDROXY COMPOUNDS; METALS; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; RADIATIONS; TRANSITION ELEMENTS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2005
Notes
PMCID: PMC1978110; PMID: 16511169; PUBLISHER-ID: za5108; OAI: oai:pubmedcentral.nih.gov:1978110