2,4-Dinitrobenzenesulfonate-functionalized carbon dots as a turn-on fluorescent probe for imaging of biothiols in living cells
Creators
- 1. Northwest University, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science (China)
- 2. Shaanxi University of Technology, Key Laboratory of Catalysis, School of Chemistry and Environment Science (China)
- 3. Northwest University, College of Life Sciences (China)
Description
The authors describe a carbon dot-based fluorescent probe for biothiols. Green emissive carbon dots (g-CDs; with λex/λem maxima of 407/505 nm) were synthesized by a one-step solvothermal method starting from 3-diethylaminophenol. They were then covalently functionalized with 2,4-dinitrobenzenesulfonyl chloride to afford 2,4-Dinitrobenzenesulfonate-functionalized CDs (g-CD-DNBS) as a nanoprobe for biothiols. The fluorescence of the g-CD-DNBS is quite weak. Upon addition of biothiols, the DNBS group of the probe is removed by thiol groups. This results in gradual restoration of the green fluorescence. The nanoprobe exhibits high selectivity for biothiols over other amino acids and biological molecules. The detection limits for cysteine, homocysteine and glutathione are 69, 74 and 69 nM (S/N = 3), respectively. The probe was applied to image biothiols in SMMC-7721 cells.
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Additional details
Identifiers
Publishing Information
- Journal Title
- Mikrochimica Acta
- Journal Volume
- 186
- Journal Issue
- 7
- Journal Page Range
- p. 1-9
- ISSN
- 0026-3672
- CODEN
- MIACAQ
INIS
- Country of Publication
- Austria
- Country of Input or Organization
- Austria
- INIS RN
- 52033582
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- ANIMAL CELLS; CARBON; CHEMICAL PREPARATION; CYSTEINE; FLUORESCENCE; GLUTATHIONE; HOMOCYSTEINE; NANOCHEMISTRY; NITRO COMPOUNDS; QUANTUM DOTS; SULFONIC ACID ESTERS
- Descriptors DEC
- AMINO ACIDS; CARBOXYLIC ACIDS; CHEMISTRY; DRUGS; ELEMENTS; EMISSION; ESTERS; LUMINESCENCE; NANOSTRUCTURES; NONMETALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PEPTIDES; PHOTON EMISSION; POLYPEPTIDES; PROTEINS; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS; SYNTHESIS; THIOLS
Optional Information
- Copyright
- Copyright (c) 2019 Springer-Verlag GmbH Austria, part of Springer Nature