Published October 20, 2006 | Version v1
Journal article Metadata-only

New crystal forms of Diocleinae lectins in the presence of different dimannosides

Creators

  • 1. Departamento de Física-IBILCE-Universidade Estadual Paulista, São José do Rio Preto 15054-000 (Brazil)
  • 2. Biomol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza-CE, Caixa Postal 6043, CEP 60455-970 (Brazil)
  • 3. Departamento de Ciências Biológicas, Universidade Regional do Cariri, Crato, CE 63195-000 (Brazil)
  • 4. Faculdade de Biociências-PUCRS, Av. Ipiranga 6681, TECNOPUC-Predio 92a, 90619-900 Porto Alegre, RS (Brazil)

Description

The crystallization and preliminary X-ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(α1-2)Man(α1)OMe, Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe in two crystal forms [the complexes with Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe crystallized in space group P32 and those with Man(α1-2)Man(α1)OMe crystallized in space group I222], which differed from those of the native proteins (P21212 for CML and C222 for CGL), are reported. Studying the interactions between lectins and sugars is important in order to explain the differences observed in the biological activities presented by the highly similar proteins of the Diocleinae subtribe. Here, the crystallization and preliminary X-ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(α1-2)Man(α1)OMe, Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe in two crystal forms [the complexes with Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe crystallized in space group P32 and those with Man(α1-2)Man(α1)OMe crystallized in space group I222], which differed from those of the native proteins (P21212 for CML and C222 for CGL), are reported. The crystal complexes of ConA-like lectins with Man(α1-4)Man(α1)OMe are reported here for the first time

Availability note (English)

Available from http://dx.doi.org/10.1107/S1744309106038887; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225211

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2225211;
DOI
10.1107/S1744309106038887;
PII
S1744309106038887;

Publishing Information

Journal Title
Acta Crystallographica. Section F
Journal Volume
62
Journal Issue
Pt 11
Journal Page Range
p. 1100-1103
ISSN
1744-3091
CODEN
ACSFCL

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46061562
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLIZATION; CRYSTALS; INTERACTIONS; PROTEINS; SACCHAROSE; SPACE GROUPS
Descriptors DEC
CARBOHYDRATES; DISACCHARIDES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; SACCHARIDES; SYMMETRY GROUPS

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2006
Notes
PMCID: PMC2225211; PMID: 17077488; PUBLISHER-ID: fw5100; OAI: oai:pubmedcentral.nih.gov:2225211