Re-canalized stenostic rabbit nasolacrimal duct by the 125I radioactive probing:Affecting Bcl-2 and Bax protein expression

To evaluate the 125I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits (New Zealand), including the nasolacrimal duct stenosis, the mechanical probing with outer layer of thermoplastic tube, and the 125I radioactive probing with the 125I seeds sealing into the thermoplastic tube. After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained, and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis. Comparing with the blank control, the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated (p<0.05), but in the 125I radioactive probing are down-/up-regulated (p<0.05) and can be used to re-canalize the stenostic lacrimal passage. The results show that the 125I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis. (authors)