Clussing and regeneration responses of various chickpea genotypes

Callussing and regeneration response were observed in some local and exotic chickpea genotypes. The brown seeded variety K850 was the best at then C44 and CM72 produced more callus masses, and were followed by black seeded variety C141. White seeded genotypes generally showed poor calussing response and among these tested, ILC195, a blight resistant variety, was the best. Calli derived from different explants belonging to various genotypes were studied for their regeneration response in many nutritional combinations. In same media, there were formation of globular structures which upon further subcultring produced callus masses only. Root formation was commonly noticed but no generation of whole plants from truly unorganized callus cells was observed. (author)