Flow injection spectrophotometric determination of ascorbic acid with 2,2'-bipyridine in anionic surfactant micellar medium

An indirect flow injection spectrophotometric method for the determination of ascorbic acid has been developed using its reducing action on iron(III) in sodium dodecyl sulfate micellar medium. Reduced iron(II) is chelated with 2,2'-bipyridine and is monitored on 520 nm. A single line FIA manifold is used throughout. The linear range of method is 10-100 micro g/ml with equation of straight-line y=-.171+0.0146x. The proposed method is rapid (sample throughput is 54 sample per hour), reproducible (RSD 0.18%) and is fairly sensitive. The procedure is successfully applied to assay ascorbic acid content in pharmaceutical preparations. (author)