Headspace volatile from rice leaves and grains

Gas chromatography - headspace method has been used to analyse volatile compounds from rice leaves and grain at different growth stages of rice plants grown at NIAB experimental farm, Faisalabad. Duncan's Multiple Range Test (DMRT) was respect to peak concentrations. The headspace chromatograms of each rice variety revealed 8 components for eaves 9 for milky and 14 for mature grains on SE-30 packed column. The genetic relationship between Basmati, DM-25 and DM-25-18-88 and their parent Basmati-370 appeared to be reflected in the composition of their volatiles. The composition of all the studied aromatic rice varieties differed mainly in the amounts of three components for eaves and milky grain and four component (ricy region) for mature grin. The preliminary results obtain so far indicate that the volatiles from the investigate varieties of rice were qualitatively similar but guantitatively different. This indicates a possible use of the headspace vapours in distinguishing among rice varieties/mutants. (author)