Radioligand assay on β-adrenoreceptor, AC and cAMP levels during myocardial ischemia
Creators
- 1. Sun Yat-Sen Univ. of Medical Sciences, Guangzhou (China)
Description
To investigate β-adrenoreceptor (β-AR) signal transduction during myocardial ischemia and the effect of Sini Decoction, the β-AR was blocked by propranolol, myocardial ischemia was induced by giving a booster dose of pituitrin in rats. The density of β-AR was detected by radioligand binding assay. The AC activity on cardiac cellular membranes and cAMP level in plasma and myocardium was detected by RIA. The results showed that the density of β-AR in myocardial ischemia groups was up-regulated (P < 0.01), but the AC activity and cAMP levels in plasma and myocardium in myocardial ischemia group were lower than normal control group (P < 0.01). The Sini Decoction (SD) can increase the density of β-AR on cardiac cellular membranes and cAMP levels in plasma (P < 0.01). Sini Decoction can reduce the desensitization of β-AR during myocardial ischemia and improve signal transduction of β-AR
Additional details
Publishing Information
- Journal Title
- Labeled Immunoassays and Clinical Medicine
- Journal Volume
- 8
- Journal Issue
- 3
- Journal Page Range
- p. 152-154
- ISSN
- 1006-1703
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 33015610
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- ADRENALINE; AMP; CARDIOVASCULAR DISEASES; CELL MEMBRANES; CYCLASES; DIAGNOSIS; IODINE 125; ISCHEMIA; LABELLED COMPOUNDS; MEDICAL EXAMINATIONS; MYOCARDIUM; RADIOIMMUNOASSAY; RECEPTORS
- Descriptors DEC
- ADRENAL HORMONES; ANEMIAS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BIOASSAY; BODY; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HEART; HEMIC DISEASES; HORMONES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LYASES; MEDICAL SURVEILLANCE; MEMBRANE PROTEINS; MEMBRANES; MUSCLES; NEUROREGULATORS; NUCLEI; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; SYMPATHOMIMETICS; SYMPTOMS; TRACER TECHNIQUES; VASCULAR DISEASES