Chromosome aberrations by a new /sup 111/In-Bleomycin complex

A new /sup 111/In-Bleomycin Complex (/sup 111/In-BLMC) was prepared and was effective for tumor imaging and therapy in mouse glioma and for killing human small cell lung cancer (SCLC) cells. Human SCLC cells (N417 or H526, NCI) were exposed to 0.9% NaCl, BLM (10-20 μg/ml) or /sup 111/In-BLMC (24-50 μCi carried by 10-20 μg BLM/ml) at 370C for 60 min. Cells were washed with fresh medium, treated with colcemid, hypotonic solution and fixer. Slides were stained with Giemsa solution and Q-binding stain. The karyotpe of the cells was analyzed. Experimental data is given. The results indicate that the /sup 111/In-BLMC causes chromosome damage which contributes to the tumor cell killing. The radiation dose from Auger electrons is small but concentrated and greatly increased chromosomal effects over that caused by BLM alone