Published 1992
| Version v1
Journal article
Specific carbon-13 labelling of leucine residues in human growth hormone
- 1. Novo Nordisk A/S, Bagsvaerd (Denmark)
- 2. Department of Chemistry, University of Copenhagen, The H.C. Oersted Institute (Denmark)
Description
Biosynthetic human growth hormone specifically 13C-labelled in the carbonyl positions of all 26 leucine residues has been obtained by recombiant DNA techniques using 13C-labelled leucine and an E. coli strain that requires leucine. It is shown that, on the whole, the labelling is specific with no significant mislabelling as would have been the case had the 13C-labelled leucine been metabolized. (au)
Additional details
Publishing Information
- Journal Title
- Acta Chemica Scandinavica (1989)
- Journal Volume
- 46
- Series
- Acta Chem. Scand. (1989).
- Journal Page Range
- 97-99
- ISSN
- 0904-213X
- CODEN
- ACHSE
INIS
- Country of Publication
- Denmark
- Country of Input or Organization
- Denmark
- INIS RN
- 23053268
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOSYNTHESIS; CARBON 13; ESCHERICHIA COLI; LABELLING; LEUCINE; MAN; NUCLEAR MAGNETIC RESONANCE; RECOMBINANT DNA; STH
- Descriptors DEC
- AMINO ACIDS; ANIMALS; BACTERIA; CARBON ISOTOPES; CARBOXYLIC ACIDS; DNA; EVEN-ODD NUCLEI; HORMONES; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PRIMATES; RESONANCE; STABLE ISOTOPES; SYNTHESIS; VERTEBRATES