Published 1975
| Version v1
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Comparison of the oxygen enhancement ratio for γ-ray-induced double-strand breaks in the DNA of bacteriophage T7 as determined by two different methods of analysis
Description
Bacteriophage T7 was irradiated in a protecting medium under nitrogen and oxygen with 60Co gamma rays. Double-strand breaks were measured by sucrose gradient sedimentation and by boundary sedimentation analysis. Both methods showed that the presence of oxygen during irradiation enhances the production of double-strand breaks. This is in contrast to a recent report which suggests that boundary sedimentation analysis does not show the effect of oxygen. The discrepancy must be ascribed to differences in the interpretation of the sedimentation data
Availability note (English)
MF available from INIS under the Report Number.Files
6217836.pdf
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Additional details
Additional titles
- Augmented title (English)
- Result interpretation
Publishing Information
- Imprint Pagination
- 19 p.
- Report number
- MBL--1975-11
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 6217836
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BACTERIOPHAGES; BIOLOGICAL RADIATION EFFECTS; CHEMICAL ANALYSIS; DNA; GAMMA RADIATION; NITROGEN; OXYGEN; RESPONSE MODIFYING FACTORS; SEDIMENTATION; SPATIAL DISTRIBUTION; STRAND BREAKS
- Descriptors DEC
- BIOLOGICAL EFFECTS; DISTRIBUTION; ELECTROMAGNETIC RADIATION; ELEMENTS; IONIZING RADIATIONS; MICROORGANISMS; NONMETALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES; RADIATION EFFECTS; RADIATIONS; VIRUSES