Published November 1983 | Version v1
Miscellaneous Metadata-only

Effects of far-ultraviolet radiation and oxygen on macromolecular synthesis and protein induction in Bacteroides fragilis BF-2

Creators

Description

The study deals with the effects of far-UV radiation, oxygen and hydrogen peroxide on macromolecular synthesis and viability in the obligate anaerobe, Bacteroides fragilis, as well as the specific proteins induced in this organism by these different DNA damaging agents. Irradiation of Bacteroides fragilis cells with far-UV light (254 nm) under anaerobic conditions resulted in the immediate, rapid and extensive degradation of DNA which continued for 40 to 60 min after irradiation. DNA degradation after irradiation was inhibited by chloramphenicol and caffeine. RNA and protein synthesis were decreased by UV irradiation and the degree of inhibition was proportional to the UV dose. Colony formation was not affected immediately by UV irradiation and continued for a dose-dependent period prior to inhibition. The relationship between the DNA damage-induced proteins, macromolecular synthesis in damaged B. fragilis cells and the observed physiological responses and inducible repair phenomena after the different DNA damaging treatments in this anaerobe are discussed

Availability note (English)

Available from the Registrar, University of Cape Town, University Private Bag, Rondebosch, 7700, South Africa.

Additional details

Publishing Information

Imprint Pagination
374 p.

INIS

Country of Publication
South Africa
Country of Input or Organization
South Africa
INIS RN
17039321
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Numerical Data, Thesis, Non-conventional Literature
Descriptors DEI
AEROBIC CONDITIONS; ANAEROBIC CONDITIONS; BACTERIA; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CAFFEINE; CARBON 14; CHLORAMPHENICOL; COLONY FORMATION; DNA; EXPERIMENTAL DATA; FAR ULTRAVIOLET RADIATION; HYDROGEN PEROXIDE; INHIBITION; IRRADIATION; LABELLING; OXYGEN; PROTEINS; RNA; SYNTHESIS
Descriptors DEC
ANALEPTICS; ANTIBIOTICS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CARBON ISOTOPES; CENTRAL NERVOUS SYSTEM AGENTS; DATA; DRUGS; ELECTROMAGNETIC RADIATION; ELEMENTS; EVEN-EVEN NUCLEI; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; INFORMATION; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NONMETALS; NUCLEI; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; OXYGEN COMPOUNDS; PEROXIDES; PURINES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; ULTRAVIOLET RADIATION; XANTHINES; YEARS LIVING RADIOISOTOPES