MgSO₄ inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC

Objective: To investigate the influence of MgSO₄ with different concentrations on cell survival and γ-H2AX expression in HUVEC irradiated with X-rays. Methods: Cell proliferation rate was assayed by CCK-8, γ-H2AX foci formation was observed with a laser confocal microscope, and γ-H2AX protein expression was detected by flow cytometer and Western blot assay. Results: MgSO₄ with a concentration of 1.25 mg/ml could improve the survival rate of HUVEC treated with X-rays (t = -6.34, P < 0.05). After irradiation, γ-H2AX foci approached to the highest level from 30 min to 1 h after radiation and then decreased. MgSO₄ significantly reduced the foci formation at 0.5, 1, 2, 6 and 12 h post-irradiation (t = 12.62, 6.36, 11.93, 5.75, 9.43, P < 0.05). Both flow cytometry and Western blot assays showed that MgSO₄ inhibited γ-H2AX protein expression at 0.5, 1 and 2 h post-irradiation (t = 6.07, 5.32, 11.85, P < 0.05). Conclusions: MgSO₄ could improve the survival rate and reduced γ-H2AX expression in HUVEC irradiated with X-ray. (authors)