Protocol for cytogenetic analysis results registration in human lymphocyte long-term cultures after radiation exposure

The proposed protocol and its computerized version allow to enhance significantly the efficacy of cytogenetic assay for evaluating the changes of aberrant cell spectrum and studying the chromosome damage transmission through several post-radiation mitoses in the cell population inhomogeneously exposed to ionizing radiation. It also promotes the elaboration of the appropriate criteria for investigating the late genetic instability on the chromosomal and sub-cellular levels