Published July 2004 | Version v1
Journal article

Dose-effect relationship between X-rays irradiation in variety of dose and CD14 expression in mouse macrophages in vivo and in vitro

  • 1. Department of Radiobiology, School of Public Health, Jilin Univ., MH Radiobiology Research Unit, Changchun (China)

Description

Objective: To study the dose-effect relationship between X-rays irradiation and CD14 expression in mouse macrophages in vivo and in vitro. Methods. The CD14 positive cells from mouse peritoneal macrophages were detected by flow cytometry (FCM) 16 hours after whole body irradiation (WBI) in vivo and from the cell line J774A.1 of mouse macrophages 4 hours after irradiation in vitro with X-rays from 0.05 Gy to 6.00 Gy. Results: WBI by low dose X-rays significantly enhanced the CD14 expression in peritoneal macrophages while the doses of 2.0 to 4.0 Gy caused an opposite effect. The X-irradiation in vitro caused a similar dose-effect curve but the suppression of CD14 expression occurred at doses of 4.0 to 6.0 Gy. Conclusion: Low dose irradiation can stimulate CD14 expression and high dose irradiation (>2.0 Gy) can suppress its expression both in vivo and in vitro

Additional details

Publishing Information

Journal Title
Journal of Jilin University. Medicine Edition
Journal Volume
30
Journal Issue
4
Journal Page Range
p. 503-505
ISSN
1671-587X